Linked Production of Pyroglutamate-Modified Proteins via Self-Cleavage of Fusion Tags with TEV Protease and Autonomous N-Terminal Cyclization with Glutaminyl Cyclase In Vivo

Open Access

Peer-reviewed

Research Article

Yan-Ping Shih,

Affiliation Institute of Biological Chemistry and Core Facilities for Protein Structural Analysis, Academia Sinica, Taipei, Taiwan
⨯
Chi-Chi Chou,

Affiliation Institute of Biological Chemistry and Core Facilities for Protein Structural Analysis, Academia Sinica, Taipei, Taiwan
⨯
Yi-Ling Chen,

Affiliation Institute of Biological Chemistry and Core Facilities for Protein Structural Analysis, Academia Sinica, Taipei, Taiwan
⨯
Kai-Fa Huang ,

* E-mail: ahjwang@gate.sinica.edu.tw (AHJW); huangkf@gate.sinica.edu.tw (KFH)

Affiliation Institute of Biological Chemistry and Core Facilities for Protein Structural Analysis, Academia Sinica, Taipei, Taiwan
⨯
Andrew H.- J. Wang

* E-mail: ahjwang@gate.sinica.edu.tw (AHJW); huangkf@gate.sinica.edu.tw (KFH)

Affiliation Institute of Biological Chemistry and Core Facilities for Protein Structural Analysis, Academia Sinica, Taipei, Taiwan
⨯

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Cell fusion
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Proteases
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Hormones
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Recombinant proteins
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Cell migration
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Glutamine
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Monocytes
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Gene cloning
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