Adenovirus-Mediated Efficient Gene Transfer into Cultured Three-Dimensional Organoids

Ning Wang; Hongyu Zhang; Bing-Qiang Zhang; Wei Liu; Zhonglin Zhang; Min Qiao; Hongmei Zhang; Fang Deng; Ningning Wu; Xian Chen; Sheng Wen; Junhui Zhang; Zhan Liao; Qian Zhang; Zhengjian Yan; Liangjun Yin; Jixing Ye; Youlin Deng; Hue H. Luu; Rex C. Haydon; Houjie Liang; Tong-Chuan He

doi:10.1371/journal.pone.0093608

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Peer-reviewed

Research Article

Adenovirus-Mediated Efficient Gene Transfer into Cultured Three-Dimensional Organoids

Ning Wang,

Affiliations Department of Oncology and Southwest Cancer Center, the Affiliated Southwest Hospital, Third Military Medical University, Chongqing, China, Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Hongyu Zhang,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Bing-Qiang Zhang,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Wei Liu,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Zhonglin Zhang,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Department of Surgery, Affiliated Zhongnan Hospital of Wuhan University, Wuhan, China
⨯
Min Qiao,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Hongmei Zhang,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Fang Deng,

Affiliation Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Ningning Wu,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Xian Chen,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Sheng Wen,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Junhui Zhang,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Zhan Liao,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Department of Orthopaedic Surgery, Xiang-Ya Hospital of Central South University, Changsha, China
⨯
Qian Zhang,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Zhengjian Yan,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Liangjun Yin,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Jixing Ye,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, School of Bioengineering, Chongqing University, Chongqing, China
⨯
Youlin Deng,

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Hue H. Luu,

Affiliation Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Rex C. Haydon,

Affiliation Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Houjie Liang ,

* E-mail: lianghoujie@sina.com (HL); tche@uchicago.edu (TCH)

Affiliation Department of Oncology and Southwest Cancer Center, the Affiliated Southwest Hospital, Third Military Medical University, Chongqing, China
⨯
[ ... ],
Tong-Chuan He

* E-mail: lianghoujie@sina.com (HL); tche@uchicago.edu (TCH)

Affiliations Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
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Adenovirus-Mediated Efficient Gene Transfer into Cultured Three-Dimensional Organoids

Ning Wang,
Hongyu Zhang,
Bing-Qiang Zhang,
Wei Liu,
Zhonglin Zhang,
Min Qiao,
Hongmei Zhang,
Fang Deng,
Ningning Wu,
Xian Chen

Published: April 2, 2014
https://doi.org/10.1371/journal.pone.0093608

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