Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

Dong Chen; Zhao Yang; Han Xia; Jun-Fu Huang; Yang Zhang; Tian-Nun Jiang; Gui-Yu Wang; Zheng-Ran Chuai; Wei-Ling Fu; Qing Huang

doi:10.1371/journal.pone.0091824

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Open Access

Peer-reviewed

Research Article

Enhanced Specificity of *TPMT**2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

Dong Chen,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Zhao Yang,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Han Xia,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Jun-Fu Huang,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Yang Zhang,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Tian-Nun Jiang,

Affiliation Department of Blood Transfusion, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Gui-Yu Wang,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Zheng-Ran Chuai,

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Wei-Ling Fu ,

* E-mail: weilingfu@yahoo.com (WLF); Dr.Q.Huang@gmail.com (QH)

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯
Qing Huang

* E-mail: weilingfu@yahoo.com (WLF); Dr.Q.Huang@gmail.com (QH)

Affiliation Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China
⨯

Enhanced Specificity of *TPMT**2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

Dong Chen,
Zhao Yang,
Han Xia,
Jun-Fu Huang,
Yang Zhang,
Tian-Nun Jiang,
Gui-Yu Wang,
Zheng-Ran Chuai,
Wei-Ling Fu,
Qing Huang

Published: April 4, 2014
https://doi.org/10.1371/journal.pone.0091824

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