Osteosarcoma Microenvironment: Whole-Slide Imaging and Optimized Antigen Detection Overcome Major Limitations in Immunohistochemical Quantification

• Pierre Kunz ,

  * E-mail: Pierre.Kunz@med.uni-heidelberg.de

  Affiliation Department of Orthopedics and Traumatology, University Hospital Heidelberg, Heidelberg, Germany

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• Jörg Fellenberg,

  Affiliation Department of Orthopedics and Traumatology, University Hospital Heidelberg, Heidelberg, Germany

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• Linda Moskovszky,

  Affiliation 1st Department of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, Budapest, Hungary

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• Zoltan Sápi,

  Affiliation 1st Department of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, Budapest, Hungary

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• Tibor Krenacs,

  Affiliation 1st Department of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, Budapest, Hungary

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• Johannes Poeschl,

  Affiliation Department of Pediatrics, University Hospital Heidelberg, Heidelberg, Germany

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• Burkhard Lehner,

  Affiliation Department of Orthopedics and Traumatology, University Hospital Heidelberg, Heidelberg, Germany

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• Miklos Szendrõi,

  Affiliation Department of Orthopedics, Semmelweis University, Budapest, Hungary

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• Volker Ewerbeck,

  Affiliation Department of Orthopedics and Traumatology, University Hospital Heidelberg, Heidelberg, Germany

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• Ralf Kinscherf,

  Affiliation Institute of Anatomy and Cell Biology, Department of Medical Cell Biology, University of Marburg, Marburg, Germany

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• Benedikt Fritzsching

  Affiliations Department of Pediatrics, University Hospital Heidelberg, Heidelberg, Germany, Department of Translational Pulmonology, Translational Lung Research Center (TLRC), Member of the German Center for Lung, University of Heidelberg, Heidelberg, Germany

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