The Use of Ovarian Cancer Cells from Patients Undergoing Surgery to Generate Primary Cultures Capable of Undergoing Functional Analysis

Rachel L. O′Donnell; Aiste McCormick; Asima Mukhopadhyay; Laura C. Woodhouse; Madeleine Moat; Anna Grundy; Michelle Dixon; Angelika Kaufman; San Soohoo; Ahmed Elattar; Nicola J. Curtin; Richard J. Edmondson

doi:10.1371/journal.pone.0090604

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Open Access

Peer-reviewed

Research Article

The Use of Ovarian Cancer Cells from Patients Undergoing Surgery to Generate Primary Cultures Capable of Undergoing Functional Analysis

Rachel L. O′Donnell ,

Contributed equally to this work with: Rachel L. O′Donnell, Aiste McCormick

Affiliations Northern Gynaecological Oncology Centre, Queen Elizabeth Hospital, Gateshead, United Kingdom, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Aiste McCormick ,

Contributed equally to this work with: Rachel L. O′Donnell, Aiste McCormick

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Asima Mukhopadhyay,

Affiliations Northern Gynaecological Oncology Centre, Queen Elizabeth Hospital, Gateshead, United Kingdom, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Laura C. Woodhouse,

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Madeleine Moat,

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Anna Grundy,

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Michelle Dixon,

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Angelika Kaufman,

Affiliations Northern Gynaecological Oncology Centre, Queen Elizabeth Hospital, Gateshead, United Kingdom, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
San Soohoo,

Affiliations Northern Gynaecological Oncology Centre, Queen Elizabeth Hospital, Gateshead, United Kingdom, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Ahmed Elattar,

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Nicola J. Curtin,

Affiliation Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
⨯
Richard J. Edmondson

* E-mail: richard.edmondson@manchester.ac.uk

Affiliation Faculty Institute for Cancer Sciences, University of Manchester, Manchester, United Kingdom
⨯

The Use of Ovarian Cancer Cells from Patients Undergoing Surgery to Generate Primary Cultures Capable of Undergoing Functional Analysis

Rachel L. O′Donnell,
Aiste McCormick,
Asima Mukhopadhyay,
Laura C. Woodhouse,
Madeleine Moat,
Anna Grundy,
Michelle Dixon,
Angelika Kaufman,
San Soohoo,
Ahmed Elattar

Published: March 6, 2014
https://doi.org/10.1371/journal.pone.0090604

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Ovarian cancer
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Ascites
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Polycystic ovary syndrome
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Transfection
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Cell cultures
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Epithelial cells
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Cancer treatment
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Immunohistochemistry techniques
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