Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase

Open Access

Peer-reviewed

Research Article

Bich Hang Do ,

Contributed equally to this work with: Bich Hang Do, Han-Bong Ryu

Affiliation Department of Physiology and Biomedical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
⨯
Han-Bong Ryu ,

Contributed equally to this work with: Bich Hang Do, Han-Bong Ryu

Affiliation Department of Physiology and Biomedical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
⨯
Phuong Hoang,

Affiliation Department of Physiology and Biomedical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Bon-Kyung Koo,

Affiliation Department of Physiology and Biomedical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
⨯
Han Choe

* E-mail: hchoe@ulsan.ac.kr

Affiliation Department of Physiology and Biomedical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Solubility
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Cell fusion
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Proteases
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Silver staining
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Protein expression
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Endotoxins
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Cytoplasm
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Sonication
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