SnapShot-Seq: A Method for Extracting Genome-Wide, In Vivo mRNA Dynamics from a Single Total RNA Sample

Jesse M. Gray; David A. Harmin; Sarah A. Boswell; Nicole Cloonan; Thomas E. Mullen; Joseph J. Ling; Nimrod Miller; Scott Kuersten; Yong-Chao Ma; Steven A. McCarroll; Sean M. Grimmond; Michael Springer

doi:10.1371/journal.pone.0089673

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Open Access

Peer-reviewed

Research Article

SnapShot-Seq: A Method for Extracting Genome-Wide, In Vivo mRNA Dynamics from a Single Total RNA Sample

Jesse M. Gray ,

Contributed equally to this work with: Jesse M. Gray, David A. Harmin

* E-mail: gray@genetics.med.harvard.edu (JMG); Michael_Springer@hms.harvard.edu (MS)

Affiliation Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
David A. Harmin ,

Contributed equally to this work with: Jesse M. Gray, David A. Harmin

Affiliation Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Sarah A. Boswell,

Affiliation Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America
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Nicole Cloonan,

Affiliation Genomic Biology Laboratory, QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia
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Thomas E. Mullen,

Affiliation Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Joseph J. Ling,

Affiliation Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Nimrod Miller,

Affiliation Departments of Pediatrics, Neurology, and Physiology, Northwestern University Feinberg School of Medicine and Lurie Children's Hospital of Chicago Research Center, Chicago, Illinois, United States of America
⨯
Scott Kuersten,

Affiliation Epicentre, Madison, Wisconsin, United States of America
⨯
Yong-Chao Ma,

Affiliation Departments of Pediatrics, Neurology, and Physiology, Northwestern University Feinberg School of Medicine and Lurie Children's Hospital of Chicago Research Center, Chicago, Illinois, United States of America
⨯
Steven A. McCarroll,

Affiliation Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Sean M. Grimmond,

Affiliation Institute for Cancer Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom
⨯
Michael Springer

* E-mail: gray@genetics.med.harvard.edu (JMG); Michael_Springer@hms.harvard.edu (MS)

Affiliation Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America
⨯

SnapShot-Seq: A Method for Extracting Genome-Wide, In Vivo mRNA Dynamics from a Single Total RNA Sample

Jesse M. Gray,
David A. Harmin,
Sarah A. Boswell,
Nicole Cloonan,
Thomas E. Mullen,
Joseph J. Ling,
Nimrod Miller,
Scott Kuersten,
Yong-Chao Ma,
Steven A. McCarroll

Published: February 26, 2014
https://doi.org/10.1371/journal.pone.0089673

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RNA sequencing
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