A Multiplex Two-Color Real-Time PCR Method for Quality-Controlled Molecular Diagnostic Testing of FFPE Samples

Jiyoun Yeo; Erin L. Crawford; Thomas M. Blomquist; Lauren M. Stanoszek; Rachel E. Dannemiller; Jill Zyrek; Luis E. De Las Casas; Sadik A. Khuder; James C. Willey

doi:10.1371/journal.pone.0089395

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Open Access

Peer-reviewed

Research Article

A Multiplex Two-Color Real-Time PCR Method for Quality-Controlled Molecular Diagnostic Testing of FFPE Samples

Jiyoun Yeo,

Affiliation Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Erin L. Crawford,

Affiliation Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Thomas M. Blomquist,

Affiliation Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Lauren M. Stanoszek,

Affiliation Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Rachel E. Dannemiller,

Affiliation Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Jill Zyrek,

Affiliation Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Luis E. De Las Casas,

Affiliation Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
Sadik A. Khuder,

Affiliation Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯
James C. Willey

* E-mail: james.willey2@utoledo.edu

Affiliations Division of Pulmonary/Critical Care and Sleep Medicine, Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America, Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America
⨯

A Multiplex Two-Color Real-Time PCR Method for Quality-Controlled Molecular Diagnostic Testing of FFPE Samples

Jiyoun Yeo,
Erin L. Crawford,
Thomas M. Blomquist,
Lauren M. Stanoszek,
Rachel E. Dannemiller,
Jill Zyrek,
Luis E. De Las Casas,
Sadik A. Khuder,
James C. Willey

Published: February 21, 2014
https://doi.org/10.1371/journal.pone.0089395

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