Prokaryotic Soluble Overexpression and Purification of Bioactive Human Growth Hormone by Fusion to Thioredoxin, Maltose Binding Protein, and Protein Disulfide Isomerase

Open Access

Peer-reviewed

Research Article

Minh Tan Nguyen ,

Contributed equally to this work with: Minh Tan Nguyen, Bon-Kyung Koo

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Bon-Kyung Koo ,

Contributed equally to this work with: Minh Tan Nguyen, Bon-Kyung Koo

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Thu Trang Thi Vu,

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Jung-A Song,

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Seon-Ha Chong,

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Boram Jeong,

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Han-Bong Ryu,

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Sang-Hyun Moh,

Affiliation Bio-FD&C Co. Ltd., Incheon, Korea
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Han Choe

* E-mail: hchoe@ulsan.ac.kr

Affiliation Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, Korea
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Solubility
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Proteases
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Disulfide bonds
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Glycerol
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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
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Cell fusion
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Growth hormone
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Plasmid construction
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