Potential Pitfalls and Solutions for Use of Fluorescent Fusion Proteins to Study the Lysosome

Ling Huang; Douglas Pike; David E. Sleat; Vikas Nanda; Peter Lobel

doi:10.1371/journal.pone.0088893

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Peer-reviewed

Research Article

Potential Pitfalls and Solutions for Use of Fluorescent Fusion Proteins to Study the Lysosome

Ling Huang,

Affiliations . Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, United States of America, Department of Pharmacology Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, United States of America
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Douglas Pike,

Affiliations . Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, United States of America, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, United States of America
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David E. Sleat,

Affiliations . Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, United States of America, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, United States of America
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Vikas Nanda,

Affiliations . Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, United States of America, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, United States of America
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Peter Lobel

* E-mail: lobel@cabm.rutgers.edu

Affiliations . Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, United States of America, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, United States of America
⨯

Potential Pitfalls and Solutions for Use of Fluorescent Fusion Proteins to Study the Lysosome

Ling Huang,
Douglas Pike,
David E. Sleat,
Vikas Nanda,
Peter Lobel

Published: February 21, 2014
https://doi.org/10.1371/journal.pone.0088893

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Lysosomes
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Cell fusion
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Transfection
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CHO cells
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Green fluorescent protein
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Signal processing
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Proteases
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