A Simple and Highly Effective Method for Slow-Freezing Human Pluripotent Stem Cells Using Dimethyl Sulfoxide, Hydroxyethyl Starch and Ethylene Glycol

Keitaro Imaizumi; Naoki Nishishita; Marie Muramatsu; Takako Yamamoto; Chiemi Takenaka; Shin Kawamata; Kenichiro Kobayashi; Shin-ichi Nishikawa; Teruo Akuta

doi:10.1371/journal.pone.0088696

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Peer-reviewed

Research Article

A Simple and Highly Effective Method for Slow-Freezing Human Pluripotent Stem Cells Using Dimethyl Sulfoxide, Hydroxyethyl Starch and Ethylene Glycol

Keitaro Imaizumi,

Current address: Kyokuto Pharmaceutical Industrial Co. Ltd., Nihonbashi Kobunecho, Chuo-ku, Tokyo, Japan

Affiliations Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan, Kobe office, RIKEN Cell Tech Co. Ltd., Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Naoki Nishishita,

Affiliations Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan, Division of Cell Therapy, Foundation for Biomedical Research and Innovation, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Marie Muramatsu,

Affiliation Division of Cell Therapy, Foundation for Biomedical Research and Innovation, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Takako Yamamoto,

Affiliation Division of Cell Therapy, Foundation for Biomedical Research and Innovation, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Chiemi Takenaka,

Affiliations Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan, Division of Cell Therapy, Foundation for Biomedical Research and Innovation, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Shin Kawamata ,

* E-mail: kawamata@fbri.org (SK); t.akuta@kyokutoseiyaku.co.jp (TA)

Affiliations Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan, Division of Cell Therapy, Foundation for Biomedical Research and Innovation, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Kenichiro Kobayashi,

Affiliations Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan, Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Okura, Setagaya-ku, Tokyo, Japan
⨯
Shin-ichi Nishikawa,

Affiliation Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯
Teruo Akuta

* E-mail: kawamata@fbri.org (SK); t.akuta@kyokutoseiyaku.co.jp (TA)

Affiliations Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan, Kobe office, RIKEN Cell Tech Co. Ltd., Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, Japan
⨯

A Simple and Highly Effective Method for Slow-Freezing Human Pluripotent Stem Cells Using Dimethyl Sulfoxide, Hydroxyethyl Starch and Ethylene Glycol

Keitaro Imaizumi,
Naoki Nishishita,
Marie Muramatsu,
Takako Yamamoto,
Chiemi Takenaka,
Shin Kawamata,
Kenichiro Kobayashi,
Shin-ichi Nishikawa,
Teruo Akuta

Published: February 12, 2014
https://doi.org/10.1371/journal.pone.0088696

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