Characterization of Diverse Internal Binding Specificities of PDZ Domains by Yeast Two-Hybrid Screening of a Special Peptide Library

Open Access

Peer-reviewed

Research Article

Yi Mu ,

Contributed equally to this work with: Yi Mu, Pengfei Cai

Affiliation National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, P.R. China
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Pengfei Cai ,

Contributed equally to this work with: Yi Mu, Pengfei Cai

Affiliation MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
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Siqi Hu,

Affiliation MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
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Sucan Ma,

Affiliation National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, P.R. China
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Youhe Gao

* E-mail: gaoyouhe@pumc.edu.cn

Affiliation National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, P.R. China
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Library screening
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Protein domains
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Sequence motif analysis
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Two-hybrid screening
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Cloning
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Peptide libraries
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Phage display
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Protein sequencing
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