A Robust High Throughput Platform to Generate Functional Recombinant Monoclonal Antibodies Using Rabbit B Cells from Peripheral Blood

Stefan Seeber; Francesca Ros; Irmgard Thorey; Georg Tiefenthaler; Klaus Kaluza; Valeria Lifke; Jens André Alexander Fischer; Stefan Klostermann; Josef Endl; Erhard Kopetzki; Achal Pashine; Basile Siewe; Brigitte Kaluza; Josef Platzer; Sonja Offner

doi:10.1371/journal.pone.0086184

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Open Access

Peer-reviewed

Research Article

A Robust High Throughput Platform to Generate Functional Recombinant Monoclonal Antibodies Using Rabbit B Cells from Peripheral Blood

Stefan Seeber,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Francesca Ros,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Irmgard Thorey,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Georg Tiefenthaler,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Klaus Kaluza,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Valeria Lifke,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Jens André Alexander Fischer,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Stefan Klostermann,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Josef Endl,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Erhard Kopetzki,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Achal Pashine,

Current address: Bristol-Myers Squibb, Immuno-Science Biology Drug Discovery, Princeton, New Jersey, United States of America

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Basile Siewe,

Current address: Rush University Medical Center, Department of Immunology and Microbiology, Chicago, Illinois, United States of America

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Brigitte Kaluza,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Josef Platzer,

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯
Sonja Offner

* E-mail: sonja.offner@roche.com

Affiliation Large Molecule Research, Pharma Research and Early Development, Roche Diagnostics GmbH, Penzberg, Germany
⨯

A Robust High Throughput Platform to Generate Functional Recombinant Monoclonal Antibodies Using Rabbit B Cells from Peripheral Blood

Stefan Seeber,
Francesca Ros,
Irmgard Thorey,
Georg Tiefenthaler,
Klaus Kaluza,
Valeria Lifke,
Jens André Alexander Fischer,
Stefan Klostermann,
Josef Endl,
Erhard Kopetzki

Published: February 4, 2014
https://doi.org/10.1371/journal.pone.0086184

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B cells
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Antibodies
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Cloning
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