Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

Claudia Lüdecke; Klaus D. Jandt; Daniel Siegismund; Marian J. Kujau; Emerson Zang; Markus Rettenmayr; Jörg Bossert; Martin Roth

doi:10.1371/journal.pone.0084837

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Peer-reviewed

Research Article

Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

Claudia Lüdecke,

Affiliations Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Bio Pilot Plant, Jena, Germany, Faculty of Physics and Astronomy, Chair of Materials Science (CMS), Friedrich Schiller University Jena, Jena, Germany
⨯
Klaus D. Jandt ,

* E-mail: martin.roth@hki-jena.de (MR); k.jandt@uni-jena.de (KDJ)

Affiliation Faculty of Physics and Astronomy, Chair of Materials Science (CMS), Friedrich Schiller University Jena, Jena, Germany
⨯
Daniel Siegismund,

Affiliation Otto Schott Institute of Materials Research (OSIM), Department of Metallic Materials, Friedrich Schiller University Jena, Jena, Germany
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Marian J. Kujau,

Current address: Wacker Biotech GmbH, Jena, Germany

Affiliation Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Bio Pilot Plant, Jena, Germany
⨯
Emerson Zang,

Affiliation Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Bio Pilot Plant, Jena, Germany
⨯
Markus Rettenmayr,

Affiliation Otto Schott Institute of Materials Research (OSIM), Department of Metallic Materials, Friedrich Schiller University Jena, Jena, Germany
⨯
Jörg Bossert,

Affiliation Faculty of Physics and Astronomy, Chair of Materials Science (CMS), Friedrich Schiller University Jena, Jena, Germany
⨯
Martin Roth

* E-mail: martin.roth@hki-jena.de (MR); k.jandt@uni-jena.de (KDJ)

Affiliation Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Bio Pilot Plant, Jena, Germany
⨯

Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

Claudia Lüdecke,
Klaus D. Jandt,
Daniel Siegismund,
Marian J. Kujau,
Emerson Zang,
Markus Rettenmayr,
Jörg Bossert,
Martin Roth

Published: January 3, 2014
https://doi.org/10.1371/journal.pone.0084837

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