Analysis of High Affinity Self-Association by Fluorescence Optical Sedimentation Velocity Analytical Ultracentrifugation of Labeled Proteins: Opportunities and Limitations

Open Access

Peer-reviewed

Research Article

Huaying Zhao,

Affiliation Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institute of Health, Bethesda, Maryland, United States of America
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Suvendu Lomash,

Affiliation Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, National Institute of Child Health and Human Development; National Institute of Health, Bethesda, Maryland, United States of America
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Carla Glasser,

Affiliation Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, National Institute of Child Health and Human Development; National Institute of Health, Bethesda, Maryland, United States of America
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Mark L. Mayer ,

* E-mail: schuckp@mail.nih.gov (PS); mayerm@mail.nih.gov (MLM)

Affiliation Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, National Institute of Child Health and Human Development; National Institute of Health, Bethesda, Maryland, United States of America
⨯
Peter Schuck

* E-mail: schuckp@mail.nih.gov (PS); mayerm@mail.nih.gov (MLM)

Affiliation Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institute of Health, Bethesda, Maryland, United States of America
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Isotherms
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Sedimentation
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Dimers
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Monomers
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Fluorescence resonance energy transfer
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Dimerization
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Hydrodynamics
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Protein interactions
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