Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines

Open Access

Peer-reviewed

Research Article

Suma Somasekharan,

Affiliation Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut, United States of America
⨯
Michelle Y. Monette,

Current address: Department of Biological and Environmental Sciences, Western Connecticut State University, Danbury, Connecticut, United States of America

Affiliation Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut, United States of America
⨯
Biff Forbush

* E-mail: biff.forbush@yale.edu

Affiliation Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut, United States of America
⨯

Reader Comments (0)

Cysteine
Is the Subject Area "Cysteine" applicable to this article?

Thanks for your feedback.
Cell membranes
Is the Subject Area "Cell membranes" applicable to this article?

Thanks for your feedback.
Yellow fluorescent protein
Is the Subject Area "Yellow fluorescent protein" applicable to this article?

Thanks for your feedback.
Membrane proteins
Is the Subject Area "Membrane proteins" applicable to this article?

Thanks for your feedback.
HEK 293 cells
Is the Subject Area "HEK 293 cells" applicable to this article?

Thanks for your feedback.
Serine
Is the Subject Area "Serine" applicable to this article?

Thanks for your feedback.
Subcloning
Is the Subject Area "Subcloning" applicable to this article?

Thanks for your feedback.
Membrane trafficking
Is the Subject Area "Membrane trafficking" applicable to this article?

Thanks for your feedback.