Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome

• Ting Shi ,

  Contributed equally to this work with: Ting Shi, Guanglu Wang

  Affiliations Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin, People’s Republic of China

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• Guanglu Wang ,

  Contributed equally to this work with: Ting Shi, Guanglu Wang

  Affiliations Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin, People’s Republic of China

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• Zhiwen Wang ,

  * E-mail: addresses: zww@tju.edu.cn

  Affiliations Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin, People’s Republic of China

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• Jing Fu,

  Affiliations Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin, People’s Republic of China

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• Tao Chen,

  Affiliations Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin, People’s Republic of China

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• Xueming Zhao

  Affiliations Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People’s Republic of China, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Tianjin University, Tianjin, People’s Republic of China

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