Rapid Assembly of Customized TALENs into Multiple Delivery Systems

Open Access

Peer-reviewed

Research Article

Zhengxing Zhang,

Current address: Department of Nephrology, Huadong Hospital, Fudan University, Shanghai, China

Affiliation Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute and Foundation, Pittsburgh, Pennsylvania, United States of America
⨯
Siliang Zhang,

Affiliation Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute and Foundation, Pittsburgh, Pennsylvania, United States of America
⨯
Xin Huang,

Affiliations Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute and Foundation, Pittsburgh, Pennsylvania, United States of America, Women’s Cancer Research Center, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, United States of America
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Kyle E. Orwig,

Affiliation Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute and Foundation, Pittsburgh, Pennsylvania, United States of America
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Yi Sheng

* E-mail: shengy@upmc.edu

Affiliation Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute and Foundation, Pittsburgh, Pennsylvania, United States of America
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TALENs
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Plasmid construction
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Transfection
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Gene targeting
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Adenoviruses
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Plasmid vectors
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Polymerase chain reaction
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Mutational analysis
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