A Combination of Let-7d, Let-7g and Let-7i Serves as a Stable Reference for Normalization of Serum microRNAs

Xi Chen; Hongwei Liang; Danping Guan; Cheng Wang; Xiaoyun Hu; Lin Cui; Sidi Chen; Chunni Zhang; Junfeng Zhang; Ke Zen; Chen-Yu Zhang

doi:10.1371/journal.pone.0079652

Loading metrics

Open Access

Peer-reviewed

Research Article

A Combination of Let-7d, Let-7g and Let-7i Serves as a Stable Reference for Normalization of Serum microRNAs

Xi Chen ,

Contributed equally to this work with: Xi Chen, Hongwei Liang, Danping Guan

Affiliation Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Hongwei Liang ,

Contributed equally to this work with: Xi Chen, Hongwei Liang, Danping Guan

Affiliation Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Danping Guan ,

Contributed equally to this work with: Xi Chen, Hongwei Liang, Danping Guan

Affiliation Nanjing MicroMedMark Biotech Co., Ltd., Nanjing, Jiangsu, China
⨯
Cheng Wang,

Affiliation Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Xiaoyun Hu,

Affiliation Nanjing MicroMedMark Biotech Co., Ltd., Nanjing, Jiangsu, China
⨯
Lin Cui,

Affiliation Nanjing MicroMedMark Biotech Co., Ltd., Nanjing, Jiangsu, China
⨯
Sidi Chen,

Affiliation Beijing MicroMedMark Biotech Co., Ltd., Beijing, China
⨯
Chunni Zhang,

Affiliation Department of Clinical Laboratory, Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing, Jiangsu, China
⨯
Junfeng Zhang ,

* E-mail: cyzhang@nju.edu.cn (CYZ); kzen@nju.edu.cn (KZ); jfzhang@nju.edu.cn (JZ)

Affiliation Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Ke Zen ,

* E-mail: cyzhang@nju.edu.cn (CYZ); kzen@nju.edu.cn (KZ); jfzhang@nju.edu.cn (JZ)

Affiliation Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯
Chen-Yu Zhang

* E-mail: cyzhang@nju.edu.cn (CYZ); kzen@nju.edu.cn (KZ); jfzhang@nju.edu.cn (JZ)

Affiliation Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
⨯

A Combination of Let-7d, Let-7g and Let-7i Serves as a Stable Reference for Normalization of Serum microRNAs

Xi Chen,
Hongwei Liang,
Danping Guan,
Cheng Wang,
Xiaoyun Hu,
Lin Cui,
Sidi Chen,
Chunni Zhang,
Junfeng Zhang,
Ke Zen

Published: November 5, 2013
https://doi.org/10.1371/journal.pone.0079652

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

MicroRNAs
Is the Subject Area "MicroRNAs" applicable to this article?

Thanks for your feedback.
Cancers and neoplasms
Is the Subject Area "Cancers and neoplasms" applicable to this article?

Thanks for your feedback.
Colorectal cancer
Is the Subject Area "Colorectal cancer" applicable to this article?

Thanks for your feedback.
Hepatocellular carcinoma
Is the Subject Area "Hepatocellular carcinoma" applicable to this article?

Thanks for your feedback.
Non-small cell lung cancer
Is the Subject Area "Non-small cell lung cancer" applicable to this article?

Thanks for your feedback.
Sedentary behavior
Is the Subject Area "Sedentary behavior" applicable to this article?

Thanks for your feedback.
RNA extraction
Is the Subject Area "RNA extraction" applicable to this article?

Thanks for your feedback.
RNA isolation
Is the Subject Area "RNA isolation" applicable to this article?

Thanks for your feedback.