Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan; Kai Zhang; Yangyan Chen; Yongjun Lin; Gaobing Wu; Lili Zhang; Pei Yao; Zongze Shao; Ziduo Liu

doi:10.1371/journal.pone.0079175

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Open Access

Peer-reviewed

Research Article

Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan,

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
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Kai Zhang,

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
⨯
Yangyan Chen,

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
⨯
Yongjun Lin,

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
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Gaobing Wu,

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
⨯
Lili Zhang,

Affiliation Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, College of Life Science, Tarim University, Alar, Xinjiang, P. R. China
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Pei Yao,

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
⨯
Zongze Shao,

Affiliation Key Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, P. R. China
⨯
Ziduo Liu

* E-mail: lzd@mail.hzau.edu.cn

Affiliation State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China
⨯

Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan,
Kai Zhang,
Yangyan Chen,
Yongjun Lin,
Gaobing Wu,
Lili Zhang,
Pei Yao,
Zongze Shao,
Ziduo Liu

Published: November 5, 2013
https://doi.org/10.1371/journal.pone.0079175

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Glycine
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Mutagenesis
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Polymerase chain reaction
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Enzymes
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DNA recombination
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Directed evolution
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Library screening
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Site-directed mutagenesis
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