Single, Double and Quadruple Alanine Substitutions at Oligomeric Interfaces Identify Hydrophobicity as the Key Determinant of Human Neutrophil Alpha Defensin HNP1 Function

Le Zhao; W. David Tolbert; Bryan Ericksen; Changyou Zhan; Xueji Wu; Weirong Yuan; Xu Li; Marzena Pazgier; Wuyuan Lu

doi:10.1371/journal.pone.0078937

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Research Article

Single, Double and Quadruple Alanine Substitutions at Oligomeric Interfaces Identify Hydrophobicity as the Key Determinant of Human Neutrophil Alpha Defensin HNP1 Function

Le Zhao ,

Contributed equally to this work with: Le Zhao, W. David Tolbert, Bryan Ericksen

Affiliations Translational Medicine Center, the First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, China, Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
W. David Tolbert ,

Contributed equally to this work with: Le Zhao, W. David Tolbert, Bryan Ericksen

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Bryan Ericksen ,

Contributed equally to this work with: Le Zhao, W. David Tolbert, Bryan Ericksen

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Changyou Zhan,

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Xueji Wu,

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Weirong Yuan,

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Xu Li ,

* E-mail: lixu56@mail.xjtu.edu.cn (XL); mpazgier@ihv.umaryland.edu (MP)

Affiliation Translational Medicine Center, the First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, China
⨯
Marzena Pazgier ,

* E-mail: lixu56@mail.xjtu.edu.cn (XL); mpazgier@ihv.umaryland.edu (MP)

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Wuyuan Lu

Affiliation Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯

Single, Double and Quadruple Alanine Substitutions at Oligomeric Interfaces Identify Hydrophobicity as the Key Determinant of Human Neutrophil Alpha Defensin HNP1 Function

Le Zhao,
W. David Tolbert,
Bryan Ericksen,
Changyou Zhan,
Xueji Wu,
Weirong Yuan,
Xu Li,
Marzena Pazgier,
Wuyuan Lu

Published: November 13, 2013
https://doi.org/10.1371/journal.pone.0078937

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