Optimization and Pharmacological Validation of a Leukocyte Migration Assay in Zebrafish Larvae for the Rapid In Vivo Bioactivity Analysis of Anti-Inflammatory Secondary Metabolites

• María Lorena Cordero-Maldonado ,

  Contributed equally to this work with: María Lorena Cordero-Maldonado, Dany Siverio-Mota

  Affiliations Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, Faculty of Chemical Sciences, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg

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• Dany Siverio-Mota ,

  Contributed equally to this work with: María Lorena Cordero-Maldonado, Dany Siverio-Mota

  Affiliations Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, Department of Pharmacy, Faculty of Chemistry-Pharmacy, Central University “Marta Abreu” of Las Villas, Santa Clara, Cuba

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• Liliana Vicet-Muro,

  Affiliations Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, Department of Pharmacy, Faculty of Chemistry-Pharmacy, Central University “Marta Abreu” of Las Villas, Santa Clara, Cuba

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• Isabel María Wilches-Arizábala,

  Affiliation Faculty of Chemical Sciences, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador

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• Camila V. Esguerra,

  Affiliation Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium

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• Peter A. M. de Witte,

  Affiliation Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium

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• Alexander D. Crawford

  * E-mail: alexander.crawford@pharm.kuleuven.be

  Affiliations Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg

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