Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

Valencio Salema; Elvira Marín; Rocio Martínez-Arteaga; David Ruano-Gallego; Sofía Fraile; Yago Margolles; Xema Teira; Carlos Gutierrez; Gustavo Bodelón; Luis Ángel Fernández

doi:10.1371/journal.pone.0075126

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Open Access

Peer-reviewed

Research Article

Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

Valencio Salema ,

Contributed equally to this work with: Valencio Salema, Elvira Marín

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Elvira Marín ,

Contributed equally to this work with: Valencio Salema, Elvira Marín

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Rocio Martínez-Arteaga,

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
David Ruano-Gallego,

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Sofía Fraile,

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Yago Margolles,

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Xema Teira,

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Carlos Gutierrez,

Affiliation Department of Animal Medicine and Surgery, Veterinary Faculty, Universidad de Las Palmas de Gran Canaria (UPGC), Las Palmas, Canary Islands, Spain
⨯
Gustavo Bodelón,

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯
Luis Ángel Fernández

* E-mail: lafdez@cnb.csic.es

Affiliation Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM Cantoblanco, Madrid, Spain
⨯

Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

Valencio Salema,
Elvira Marín,
Rocio Martínez-Arteaga,
David Ruano-Gallego,
Sofía Fraile,
Yago Margolles,
Xema Teira,
Carlos Gutierrez,
Gustavo Bodelón,
Luis Ángel Fernández

Published: September 23, 2013
https://doi.org/10.1371/journal.pone.0075126

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