Next-Generation Sequencing of HIV-1 RNA Genomes: Determination of Error Rates and Minimizing Artificial Recombination

Francesca Di Giallonardo; Osvaldo Zagordi; Yannick Duport; Christine Leemann; Beda Joos; Marzanna Künzli-Gontarczyk; Rémy Bruggmann; Niko Beerenwinkel; Huldrych F. Günthard; Karin J. Metzner

doi:10.1371/journal.pone.0074249

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Open Access

Peer-reviewed

Research Article

Next-Generation Sequencing of HIV-1 RNA Genomes: Determination of Error Rates and Minimizing Artificial Recombination

Francesca Di Giallonardo ,

Contributed equally to this work with: Francesca Di Giallonardo, Osvaldo Zagordi

Affiliation Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
⨯
Osvaldo Zagordi ,

Contributed equally to this work with: Francesca Di Giallonardo, Osvaldo Zagordi

Affiliations Department of Biosystems Sciences and Engineering, ETH Zurich, Basel, Switzerland, Institute of Medical Virology, University of Zurich, Zurich, Switzerland
⨯
Yannick Duport,

Affiliation Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
⨯
Christine Leemann,

Affiliation Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
⨯
Beda Joos,

Affiliation Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
⨯
Marzanna Künzli-Gontarczyk,

Affiliation Functional Genomics Center Zurich (FGCZ), University of Zurich, ETH Zurich, Zurich, Switzerland
⨯
Rémy Bruggmann,

Current address: Department of Biology, Bioinformatics, University of Bern, Bern, Switzerland

Affiliation Functional Genomics Center Zurich (FGCZ), University of Zurich, ETH Zurich, Zurich, Switzerland
⨯
Niko Beerenwinkel,

Affiliations Department of Biosystems Sciences and Engineering, ETH Zurich, Basel, Switzerland, SIB Swiss Institute of Bioinformatics, Basel, Switzerland
⨯
Huldrych F. Günthard,

Affiliation Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
⨯
Karin J. Metzner

* E-mail: karin.metzner@usz.ch

Affiliation Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland
⨯

Next-Generation Sequencing of HIV-1 RNA Genomes: Determination of Error Rates and Minimizing Artificial Recombination

Francesca Di Giallonardo,
Osvaldo Zagordi,
Yannick Duport,
Christine Leemann,
Beda Joos,
Marzanna Künzli-Gontarczyk,
Rémy Bruggmann,
Niko Beerenwinkel,
Huldrych F. Günthard,
Karin J. Metzner

Published: September 18, 2013
https://doi.org/10.1371/journal.pone.0074249

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Polymerase chain reaction
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HIV-1
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Haplotypes
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Next-generation sequencing
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DNA recombination
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Oligonucleotides
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