Directed Evolution of Mycobacterium tuberculosis β-Lactamase Reveals Gatekeeper Residue That Regulates Antibiotic Resistance and Catalytic Efficiency

• Christian Feiler ,

  Contributed equally to this work with: Christian Feiler, Adam C. Fisher

  Affiliation School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America

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• Adam C. Fisher ,

  Contributed equally to this work with: Christian Feiler, Adam C. Fisher

  Affiliation School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America

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• Jason T. Boock,

  Affiliation School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America

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• Matthew J. Marrichi,

  Affiliation School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America

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• Lori Wright,

  Affiliation Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America

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• Philipp A. M. Schmidpeter,

  Affiliation Laboratorium für Biochemie und Bayreuther Zentrum für Molekulare Biowissenschaften, Universität Bayreuth, Bayreuth, Germany

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• Wulf Blankenfeldt,

  Affiliation Laboratorium für Biochemie und Bayreuther Zentrum für Molekulare Biowissenschaften, Universität Bayreuth, Bayreuth, Germany

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• Martin Pavelka,

  Affiliation Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America

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• Matthew P. DeLisa

  * E-mail: md255@cornell.edu

  Affiliation School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America

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