Cold Adaptation, Ca2+ Dependency and Autolytic Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications

Open Access

Peer-reviewed

Research Article

Cold Adaptation, Ca²⁺ Dependency and Autolytic Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications

Alvaro Olivera-Nappa ,

* E-mail: aolivera@ing.uchile.cl

Affiliation Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, University of Chile, Santiago, Chile
⨯
Fernando Reyes,

Affiliation Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, University of Chile, Santiago, Chile
⨯
Barbara A. Andrews,

Affiliation Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, University of Chile, Santiago, Chile
⨯
Juan A. Asenjo

Affiliation Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, University of Chile, Santiago, Chile
⨯

Serine proteases
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Swine
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Autolysis
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Enzymes
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Proteases
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Protein sequencing
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Recombinant protein purification
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Recombinant proteins
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