A Method for 3D Immunostaining and Optical Imaging of the Mouse Brain Demonstrated in Neural Progenitor Cells

Open Access

Peer-reviewed

Research Article

Jacqueline A. Gleave,

Affiliations Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada, Department of Biological Sciences, University of Toronto, Toronto, Ontario, Canada
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Jason P. Lerch,

Affiliations Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
⨯
R. Mark Henkelman,

Affiliations Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
⨯
Brian J. Nieman

* E-mail: bjnieman@mouseimaging.ca

Affiliations Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
⨯

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Cell staining
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Immunohistochemistry techniques
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Neuroimaging
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Serial two-photon tomography
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Nestin
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Staining
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Immunostaining
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Imaging techniques
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