Accurate Detection and Quantification of the Fish Viral Hemorrhagic Septicemia virus (VHSv) with a Two-Color Fluorometric Real-Time PCR Assay

• Lindsey R. Pierce ,

  Contributed equally to this work with: Lindsey R. Pierce, James C. Willey

  Affiliation Great Lakes Genetics/Genomics Laboratory, Lake Erie Center and Department of Environmental Sciences, The University of Toledo, Toledo, Ohio, United States of America

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• James C. Willey ,

  Contributed equally to this work with: Lindsey R. Pierce, James C. Willey

  Affiliation Department of Medicine, The University of Toledo, Toledo, Ohio, United States of America

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• Vrushalee V. Palsule,

  Affiliation Great Lakes Genetics/Genomics Laboratory, Lake Erie Center and Department of Environmental Sciences, The University of Toledo, Toledo, Ohio, United States of America

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• Jiyoun Yeo,

  Affiliation Department of Medicine, The University of Toledo, Toledo, Ohio, United States of America

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• Brian S. Shepherd,

  Affiliation ARS/USDA/University of Wisconsin at Milwaukee/School of Freshwater Sciences, Milwaukee, Wisconsin, United States of America

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• Erin L. Crawford,

  Affiliation Department of Medicine, The University of Toledo, Toledo, Ohio, United States of America

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• Carol A. Stepien

  * E-mail: carol.stepien@utoledo.edu

  Affiliation Great Lakes Genetics/Genomics Laboratory, Lake Erie Center and Department of Environmental Sciences, The University of Toledo, Toledo, Ohio, United States of America

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