Impact of Library Preparation on Downstream Analysis and Interpretation of RNA-Seq Data: Comparison between Illumina PolyA and NuGEN Ovation Protocol

Zhifu Sun; Yan W. Asmann; Asha Nair; Yuji Zhang; Liguo Wang; Krishna R. Kalari; Aditya V. Bhagwate; Tiffany R. Baker; Jennifer M. Carr; Jean-Pierre A. Kocher; Edith A. Perez; E. Aubrey Thompson

doi:10.1371/journal.pone.0071745

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Open Access

Peer-reviewed

Research Article

Impact of Library Preparation on Downstream Analysis and Interpretation of RNA-Seq Data: Comparison between Illumina PolyA and NuGEN Ovation Protocol

Zhifu Sun ,

* E-mail: sun.zhifu@mayo.edu (ZS); thompson.aubrey@mayo.edu (EAT)

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Yan W. Asmann,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Asha Nair,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Yuji Zhang,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Liguo Wang,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Krishna R. Kalari,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Aditya V. Bhagwate,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Tiffany R. Baker,

Affiliation Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Jacksonville, Florida, United States of America
⨯
Jennifer M. Carr,

Affiliation Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Jacksonville, Florida, United States of America
⨯
Jean-Pierre A. Kocher,

Affiliation Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
⨯
Edith A. Perez,

Affiliation Department of Medicine, Mayo Clinic, Jacksonville, Florida, United States of America
⨯
E. Aubrey Thompson

* E-mail: sun.zhifu@mayo.edu (ZS); thompson.aubrey@mayo.edu (EAT)

Affiliation Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Jacksonville, Florida, United States of America
⨯

Impact of Library Preparation on Downstream Analysis and Interpretation of RNA-Seq Data: Comparison between Illumina PolyA and NuGEN Ovation Protocol

Zhifu Sun,
Yan W. Asmann,
Asha Nair,
Yuji Zhang,
Liguo Wang,
Krishna R. Kalari,
Aditya V. Bhagwate,
Tiffany R. Baker,
Jennifer M. Carr,
Jean-Pierre A. Kocher

Published: August 19, 2013
https://doi.org/10.1371/journal.pone.0071745

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Gene expression
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Long non-coding RNA
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RNA sequencing
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cDNA libraries
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Sequence alignment
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Exon mapping
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Genome annotation
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Reverse transcriptase-polymerase chain reaction
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