The Effect of Primer Choice and Short Read Sequences on the Outcome of 16S rRNA Gene Based Diversity Studies

Open Access

Peer-reviewed

Research Article

Jonas Ghyselinck ,

Contributed equally to this work with: Jonas Ghyselinck, Stefan Pfeiffer

* E-mail: jonas_ghyselinck@hotmail.com (JG); stefan.pfeiffer.fl@ait.ac.at (SP)

Affiliation Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
⨯
Stefan Pfeiffer ,

Contributed equally to this work with: Jonas Ghyselinck, Stefan Pfeiffer

* E-mail: jonas_ghyselinck@hotmail.com (JG); stefan.pfeiffer.fl@ait.ac.at (SP)

Affiliation Department of Health and Environment, Bioresources Unit, AIT Austrian Institute of Technology GmbH, Tulln, Austria
⨯
Kim Heylen,

Affiliation Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
⨯
Angela Sessitsch,

Affiliation Department of Health and Environment, Bioresources Unit, AIT Austrian Institute of Technology GmbH, Tulln, Austria
⨯
Paul De Vos

Affiliation Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
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Phylogenetic analysis
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Ribosomal RNA
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Phylogenetics
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Taxonomy
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Gene sequencing
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Trees
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Sequence alignment
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Bacterial taxonomy
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