Development and Validation of a Multiplex, Real-Time RT PCR Assay for the Simultaneous Detection of Classical and African Swine Fever Viruses

Open Access

Peer-reviewed

Research Article

Felicity J. Haines,

Affiliation Virology Department, Animal Health and Veterinary Laboratories Agency, New Haw, Surrey, United Kingdom
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Martin A. Hofmann,

Affiliation Development Department, Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland
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Donald P. King,

Affiliation The Pirbright Institute, Pirbright Laboratory, Pirbright, Surrey, United Kingdom
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Trevor W. Drew,

Affiliation Virology Department, Animal Health and Veterinary Laboratories Agency, New Haw, Surrey, United Kingdom
⨯
Helen R. Crooke

* E-mail: Helen.Crooke@ahvla.gsi.gov.uk

Affiliation Virology Department, Animal Health and Veterinary Laboratories Agency, New Haw, Surrey, United Kingdom
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Reverse transcriptase-polymerase chain reaction
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Polymerase chain reaction
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Nucleic acids
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Swine
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Classical swine fever
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DNA isolation
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Viral nucleic acid
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Optimization
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