Generation of Live Piglets for the First Time Using Sperm Retrieved from Immature Testicular Tissue Cryopreserved and Grafted into Nude Mice

• Hiroyuki Kaneko ,

  Contributed equally to this work with: Hiroyuki Kaneko, Kazuhiro Kikuchi

  Affiliation Animal Development and Differentiation Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan

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• Kazuhiro Kikuchi ,

  Contributed equally to this work with: Hiroyuki Kaneko, Kazuhiro Kikuchi

  * E-mail: kiku@affrc.go.jp

  Affiliations Animal Development and Differentiation Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan

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• Michiko Nakai,

  Affiliation Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan

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• Tamas Somfai,

  Affiliation Animal Breeding and Reproduction Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan

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• Junko Noguchi,

  Affiliation Animal Development and Differentiation Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan

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• Fuminori Tanihara,

  Affiliation The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan

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• Junya Ito,

  Affiliation Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan

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• Naomi Kashiwazaki

  Affiliation Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan

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