A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

Hussam H. Shaheen; Bianka Prinz; Ming-Tang Chen; Tej Pavoor; Song Lin; Nga Rewa Houston-Cummings; Renee Moore; Terrance A. Stadheim; Dongxing Zha

doi:10.1371/journal.pone.0070190

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Open Access

Peer-reviewed

Research Article

A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

Hussam H. Shaheen ,

* E-mail: hussam.shaheen@merck.com (HS); dongxing.zha@merck.com (DZ)

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Bianka Prinz,

Current address: Adimab, Lebanon, New Hampshire, United States of America

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Ming-Tang Chen,

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Tej Pavoor,

Current address: New York, New York, United States of America

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Song Lin,

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Nga Rewa Houston-Cummings,

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Renee Moore,

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Terrance A. Stadheim,

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯
Dongxing Zha

* E-mail: hussam.shaheen@merck.com (HS); dongxing.zha@merck.com (DZ)

Affiliation GlycoFi, Biologics Discovery, Merck Research Laboratories, Merck & Co., Inc., Lebanon, New Hampshire, United States of America
⨯

A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

Hussam H. Shaheen,
Bianka Prinz,
Ming-Tang Chen,
Tej Pavoor,
Song Lin,
Nga Rewa Houston-Cummings,
Renee Moore,
Terrance A. Stadheim,
Dongxing Zha

Published: July 10, 2013
https://doi.org/10.1371/journal.pone.0070190

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Pichia pastoris
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Flow cytometry
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