LC-MS/MS Confirms That COX-1 Drives Vascular Prostacyclin Whilst Gene Expression Pattern Reveals Non-Vascular Sites of COX-2 Expression

Nicholas S. Kirkby; Anne K. Zaiss; Paula Urquhart; Jing Jiao; Philip J. Austin; Malak Al-Yamani; Martina H. Lundberg; Louise S. MacKenzie; Timothy D. Warner; Anna Nicolaou; Harvey R. Herschman; Jane A. Mitchell

doi:10.1371/journal.pone.0069524

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Peer-reviewed

Research Article

LC-MS/MS Confirms That COX-1 Drives Vascular Prostacyclin Whilst Gene Expression Pattern Reveals Non-Vascular Sites of COX-2 Expression

Nicholas S. Kirkby ,

* E-mail: j.a.mitchell@imperial.ac.uk (JAM); n.kirkby@imperial.ac.uk (NSK)

Affiliations National Heart & Lung Institute, Imperial College London, London, United Kingdom, The William Harvey Research Institute, Barts & the London School of Medicine & Dentistry, Queen Mary University of London, London, United Kingdom
⨯
Anne K. Zaiss,

Affiliation Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America
⨯
Paula Urquhart,

Affiliation School of Pharmacy, University of Bradford, Bradford, United Kingdom
⨯
Jing Jiao,

Affiliation Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America
⨯
Philip J. Austin,

Affiliation National Heart & Lung Institute, Imperial College London, London, United Kingdom
⨯
Malak Al-Yamani,

Affiliations National Heart & Lung Institute, Imperial College London, London, United Kingdom, King Fahad Cardiac Center of King Saud University, Riyadh, Saudi Arabia
⨯
Martina H. Lundberg,

Affiliation The William Harvey Research Institute, Barts & the London School of Medicine & Dentistry, Queen Mary University of London, London, United Kingdom
⨯
Louise S. MacKenzie,

Affiliation School of Life and Medical Sciences, University of Hertfordshire, Hertfordshire, United Kingdom
⨯
Timothy D. Warner,

Affiliation The William Harvey Research Institute, Barts & the London School of Medicine & Dentistry, Queen Mary University of London, London, United Kingdom
⨯
Anna Nicolaou,

Affiliation School of Pharmacy, University of Bradford, Bradford, United Kingdom
⨯
Harvey R. Herschman,

Affiliation Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America
⨯
Jane A. Mitchell

* E-mail: j.a.mitchell@imperial.ac.uk (JAM); n.kirkby@imperial.ac.uk (NSK)

Affiliation National Heart & Lung Institute, Imperial College London, London, United Kingdom
⨯

LC-MS/MS Confirms That COX-1 Drives Vascular Prostacyclin Whilst Gene Expression Pattern Reveals Non-Vascular Sites of COX-2 Expression

Nicholas S. Kirkby,
Anne K. Zaiss,
Paula Urquhart,
Jing Jiao,
Philip J. Austin,
Malak Al-Yamani,
Martina H. Lundberg,
Louise S. MacKenzie,
Timothy D. Warner,
Anna Nicolaou

Published: July 9, 2013
https://doi.org/10.1371/journal.pone.0069524

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Prostacyclin
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Aorta
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Gene expression
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Immunoassays
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Deletion mutation
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Luciferase
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COX-2 inhibitors
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Blood plasma
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