An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli

Open Access

Peer-reviewed

Research Article

Johannes Schiffels,

Affiliation Department of Chemistry and Biotechnology, Aachen University of Applied Sciences, Juelich, Germany
⨯
Olaf Pinkenburg,

Affiliation Institute for Immunology, Biomedical Research Centre (BMFZ), Philipps University of Marburg, Marburg (Lahn), Germany
⨯
Maximilian Schelden,

Affiliation Department of Chemistry and Biotechnology, Aachen University of Applied Sciences, Juelich, Germany
⨯
El-Hussiny A. A. Aboulnaga,

Affiliation Department of Chemistry and Biotechnology, Aachen University of Applied Sciences, Juelich, Germany
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Marcus E. M. Baumann,

Affiliation Department of Chemistry and Biotechnology, Aachen University of Applied Sciences, Juelich, Germany
⨯
Thorsten Selmer

* E-mail: selmer@fh-aachen.de

Affiliation Department of Chemistry and Biotechnology, Aachen University of Applied Sciences, Juelich, Germany
⨯

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Plasmid construction
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Nickel
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Necator
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Gene cloning
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Structural genes
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Cloning
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Gene expression
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Oxygen
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