Rapid Detection and Simultaneous Genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in Powdered Infant Formula Using Real-time PCR and High Resolution Melting (HRM) Analysis

Xian-Quan Cai; Hai-Qiong Yu; Zhou-Xi Ruan; Lei-Liang Yang; Jian-Shan Bai; De-Yi Qiu; Zhi-Hua Jian; Yi-Qian Xiao; Jie-Yang Yang; Thanh Hoa Le; Xing-Quan Zhu

doi:10.1371/journal.pone.0067082

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Open Access

Peer-reviewed

Research Article

Rapid Detection and Simultaneous Genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in Powdered Infant Formula Using Real-time PCR and High Resolution Melting (HRM) Analysis

Xian-Quan Cai,

Affiliations State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, People’s Republic of China, Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong Province, People’s Republic of China
⨯
Hai-Qiong Yu,

Affiliation Technical Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, Guangdong Province, People’s Republic of China
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Zhou-Xi Ruan,

Affiliation Animal & Plant Inspection and Quarantine Technical Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, Guangdong Province, People’s Republic of China
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Lei-Liang Yang,

Affiliation Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong Province, People’s Republic of China
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Jian-Shan Bai,

Affiliation Guangzhou Airport Entry-Exit Inspection and Quarantine Bureau, Guangzhou, Guangdong Province, People’s Republic of China
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De-Yi Qiu,

Affiliation Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong Province, People’s Republic of China
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Zhi-Hua Jian,

Affiliation Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong Province, People’s Republic of China
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Yi-Qian Xiao,

Affiliation Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong Province, People’s Republic of China
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Jie-Yang Yang,

Affiliation Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong Province, People’s Republic of China
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Thanh Hoa Le,

Affiliation Immunology Department, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
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Xing-Quan Zhu

* E-mail: xingquanzhu1@hotmail.com

Affiliation State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, People’s Republic of China
⨯

Rapid Detection and Simultaneous Genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in Powdered Infant Formula Using Real-time PCR and High Resolution Melting (HRM) Analysis

Xian-Quan Cai,
Hai-Qiong Yu,
Zhou-Xi Ruan,
Lei-Liang Yang,
Jian-Shan Bai,
De-Yi Qiu,
Zhi-Hua Jian,
Yi-Qian Xiao,
Jie-Yang Yang,
Thanh Hoa Le

Published: June 25, 2013
https://doi.org/10.1371/journal.pone.0067082

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