Evaluating the Impact of Sequencing Depth on Transcriptome Profiling in Human Adipose

Yichuan Liu; Jane F. Ferguson; Chenyi Xue; Ian M. Silverman; Brian Gregory; Muredach P. Reilly; Mingyao Li

doi:10.1371/journal.pone.0066883

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Peer-reviewed

Research Article

Evaluating the Impact of Sequencing Depth on Transcriptome Profiling in Human Adipose

Yichuan Liu,

Affiliation Department of Biostatistics and Epidemiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Jane F. Ferguson,

Affiliation Cardiovascular Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Chenyi Xue,

Affiliation Cardiovascular Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Ian M. Silverman,

Affiliation Department of Biology, Perelman School of Medicine and School of Arts and Science at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Brian Gregory,

Affiliation Department of Biology, Perelman School of Medicine and School of Arts and Science at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Muredach P. Reilly ,

Contributed equally to this work with: Muredach P. Reilly, Mingyao Li

* E-mail: mingyao@mail.med.upenn.edu (ML); muredach@mail.med.upenn.edu (MPR)

Affiliation Cardiovascular Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Mingyao Li

Contributed equally to this work with: Muredach P. Reilly, Mingyao Li

* E-mail: mingyao@mail.med.upenn.edu (ML); muredach@mail.med.upenn.edu (MPR)

Affiliation Department of Biostatistics and Epidemiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯

Evaluating the Impact of Sequencing Depth on Transcriptome Profiling in Human Adipose

Yichuan Liu,
Jane F. Ferguson,
Chenyi Xue,
Ian M. Silverman,
Brian Gregory,
Muredach P. Reilly,
Mingyao Li

Published: June 24, 2013
https://doi.org/10.1371/journal.pone.0066883

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Gene expression
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Gene sequencing
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RNA sequencing
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Transcriptome analysis
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Alternative splicing
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Blood
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Sequence alignment
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