Engineered External Guide Sequences Are Highly Effective in Inhibiting Gene Expression and Replication of Hepatitis B Virus in Cultured Cells

Zhigang Zhang; Gia-Phong Vu; Hao Gong; Chuan Xia; Yuan-Chuan Chen; Fenyong Liu; Jianguo Wu; Sangwei Lu

doi:10.1371/journal.pone.0065268

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Peer-reviewed

Research Article

Engineered External Guide Sequences Are Highly Effective in Inhibiting Gene Expression and Replication of Hepatitis B Virus in Cultured Cells

Zhigang Zhang ,

Contributed equally to this work with: Zhigang Zhang, Gia-Phong Vu, Hao Gong

Affiliation State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China
⨯
Gia-Phong Vu ,

Contributed equally to this work with: Zhigang Zhang, Gia-Phong Vu, Hao Gong

Affiliation Program in Comparative Biochemistry, University of California, Berkeley, California, United States of America
⨯
Hao Gong ,

Contributed equally to this work with: Zhigang Zhang, Gia-Phong Vu, Hao Gong

Affiliation School of Public Health, University of California, Berkeley, California, United States of America
⨯
Chuan Xia,

Affiliation State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China
⨯
Yuan-Chuan Chen,

Affiliation Program in Comparative Biochemistry, University of California, Berkeley, California, United States of America
⨯
Fenyong Liu ,

* E-mail: liu_fy@berkeley.edu (FL); sangwei@berkeley.edu (SW); jwu@whu.edu.cn (JW)

Affiliations State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China, Program in Comparative Biochemistry, University of California, Berkeley, California, United States of America, School of Public Health, University of California, Berkeley, California, United States of America
⨯
Jianguo Wu ,

* E-mail: liu_fy@berkeley.edu (FL); sangwei@berkeley.edu (SW); jwu@whu.edu.cn (JW)

Affiliation State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China
⨯
Sangwei Lu

* E-mail: liu_fy@berkeley.edu (FL); sangwei@berkeley.edu (SW); jwu@whu.edu.cn (JW)

Affiliations Program in Comparative Biochemistry, University of California, Berkeley, California, United States of America, School of Public Health, University of California, Berkeley, California, United States of America
⨯

Engineered External Guide Sequences Are Highly Effective in Inhibiting Gene Expression and Replication of Hepatitis B Virus in Cultured Cells

Zhigang Zhang,
Gia-Phong Vu,
Hao Gong,
Chuan Xia,
Yuan-Chuan Chen,
Fenyong Liu,
Jianguo Wu,
Sangwei Lu

Published: June 12, 2013
https://doi.org/10.1371/journal.pone.0065268

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Ribonucleases
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Salmonella
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Hepatitis B virus
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Transfer RNA
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Cell cultures
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Gene expression
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