Pichia pastoris-Expressed Dengue 2 Envelope Forms Virus-Like Particles without Pre-Membrane Protein and Induces High Titer Neutralizing Antibodies

Shailendra Mani; Lav Tripathi; Rajendra Raut; Poornima Tyagi; Upasana Arora; Tarani Barman; Ruchi Sood; Alka Galav; Wahala Wahala; Aravinda de Silva; Sathyamangalam Swaminathan; Navin Khanna

doi:10.1371/journal.pone.0064595

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Open Access

Peer-reviewed

Research Article

Pichia pastoris-Expressed Dengue 2 Envelope Forms Virus-Like Particles without Pre-Membrane Protein and Induces High Titer Neutralizing Antibodies

Shailendra Mani ,

Contributed equally to this work with: Shailendra Mani, Lav Tripathi

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Lav Tripathi ,

Contributed equally to this work with: Shailendra Mani, Lav Tripathi

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Rajendra Raut,

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Poornima Tyagi,

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Upasana Arora,

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Tarani Barman,

Affiliation Department of Infectious Diseases, Ranbaxy Research Laboratories, Udyog Vihar, Gurgaon, Haryana, India
⨯
Ruchi Sood,

Affiliation Department of Infectious Diseases, Ranbaxy Research Laboratories, Udyog Vihar, Gurgaon, Haryana, India
⨯
Alka Galav,

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Wahala Wahala,

Affiliation Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America
⨯
Aravinda de Silva,

Affiliation Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America
⨯
Sathyamangalam Swaminathan ,

* E-mail: swami@icgeb.res.in (SS); navin@icgeb.res.in (NK)

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯
Navin Khanna

* E-mail: swami@icgeb.res.in (SS); navin@icgeb.res.in (NK)

Affiliation Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
⨯

Pichia pastoris-Expressed Dengue 2 Envelope Forms Virus-Like Particles without Pre-Membrane Protein and Induces High Titer Neutralizing Antibodies

Shailendra Mani,
Lav Tripathi,
Rajendra Raut,
Poornima Tyagi,
Upasana Arora,
Tarani Barman,
Ruchi Sood,
Alka Galav,
Wahala Wahala,
Aravinda de Silva

Published: May 23, 2013
https://doi.org/10.1371/journal.pone.0064595

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