A Robust Strategy for Negative Selection of Cre-LoxP Recombination-Based Excision of Transgenes in Induced Pluripotent Stem Cells

Open Access

Peer-reviewed

Research Article

Syandan Chakraborty,

Affiliation Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America
⨯
Nicolas Christoforou,

Affiliation Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America
⨯
Ali Fattahi,

Affiliation Department of Pharmaceutics, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
⨯
Roland W. Herzog,

Affiliation Department of Pediatrics, University of Florida, Gainesville, Florida, United States of America
⨯
Kam W. Leong

* E-mail: kam.leong@duke.edu

Affiliation Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America
⨯

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Cell differentiation
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Induced pluripotent stem cells
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Cloning
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Pluripotency
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Reverse transcriptase-polymerase chain reaction
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Cell staining
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Plasmid construction
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Neuronal differentiation
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