Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71

Xing Wu; Qunying Mao; Xin Yao; Pan Chen; Xiangmei Chen; Jie Shao; Fan Gao; Xiang Yu; Fengcai Zhu; Rongcheng Li; Wenhui Li; Zhenglun Liang; Junzhi Wang; Fengmin Lu

doi:10.1371/journal.pone.0064116

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Open Access

Peer-reviewed

Research Article

Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71

Xing Wu,

Affiliations Department of Microbiology and Infectious Disease Center, Peking University Health Science Center, Beijing, China, Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Qunying Mao,

Affiliation Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Xin Yao,

Affiliation Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Pan Chen,

Affiliation National Institute of Biological Sciences, Beijing, China
⨯
Xiangmei Chen,

Affiliation Department of Microbiology and Infectious Disease Center, Peking University Health Science Center, Beijing, China
⨯
Jie Shao,

Affiliation Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Fan Gao,

Affiliation Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Xiang Yu,

Affiliation Hualan Biological Engineering Inc. , Henan, China
⨯
Fengcai Zhu,

Affiliation Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China
⨯
Rongcheng Li,

Affiliation The Center for Disease Control and Prevention of the Guangxi Zhuang Automomous Region, Nanning, China
⨯
Wenhui Li,

Affiliation National Institute of Biological Sciences, Beijing, China
⨯
Zhenglun Liang ,

* E-mail: lu.fengmin@hsc.pku.edu.cn (FML); lzlun@yahoo.com (ZLL)

Affiliation Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Junzhi Wang,

Affiliation Department of Hepatitis Vaccine, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China
⨯
Fengmin Lu

* E-mail: lu.fengmin@hsc.pku.edu.cn (FML); lzlun@yahoo.com (ZLL)

Affiliation Department of Microbiology and Infectious Disease Center, Peking University Health Science Center, Beijing, China
⨯

Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71

Xing Wu,
Qunying Mao,
Xin Yao,
Pan Chen,
Xiangmei Chen,
Jie Shao,
Fan Gao,
Xiang Yu,
Fengcai Zhu,
Rongcheng Li

Published: June 5, 2013
https://doi.org/10.1371/journal.pone.0064116

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