A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

Swee Jin Tan; Huan Phan; Benjamin Michael Gerry; Alexandre Kuhn; Lewis Zuocheng Hong; Yao Min Ong; Polly Suk Yean Poon; Marc Alexander Unger; Robert C. Jones; Stephen R. Quake; William F. Burkholder

doi:10.1371/journal.pone.0064084

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Open Access

Peer-reviewed

Research Article

A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

Swee Jin Tan,

Affiliation Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore
⨯
Huan Phan,

Current address: NVS Technologies, Inc., Menlo Park, CA, USA

Affiliation Fluidigm Corporation, South San Francisco, California, United States of America
⨯
Benjamin Michael Gerry,

Affiliation Fluidigm Corporation, South San Francisco, California, United States of America
⨯
Alexandre Kuhn,

Affiliation Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore
⨯
Lewis Zuocheng Hong,

Affiliation Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore
⨯
Yao Min Ong,

Affiliation Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore
⨯
Polly Suk Yean Poon,

Affiliation Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore
⨯
Marc Alexander Unger,

Affiliation Fluidigm Corporation, South San Francisco, California, United States of America
⨯
Robert C. Jones,

Affiliation Fluidigm Corporation, South San Francisco, California, United States of America
⨯
Stephen R. Quake,

Affiliations Departments of Bioengineering and Applied Physics, Stanford University and Howard Hughes Medical Institute, Stanford, California, United States of America, Visiting Investigator, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore, Singapore
⨯
William F. Burkholder

* E-mail: wfburkholder@gmail.com

Affiliation Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Proteos, Singapore
⨯

A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

Swee Jin Tan,
Huan Phan,
Benjamin Michael Gerry,
Alexandre Kuhn,
Lewis Zuocheng Hong,
Yao Min Ong,
Polly Suk Yean Poon,
Marc Alexander Unger,
Robert C. Jones,
Stephen R. Quake

Published: July 24, 2013
https://doi.org/10.1371/journal.pone.0064084

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