Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching

Sarah R. Needham; Michael Hirsch; Daniel J. Rolfe; David T. Clarke; Laura C. Zanetti-Domingues; Richard Wareham; Marisa L. Martin-Fernandez

doi:10.1371/journal.pone.0062331

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Research Article

Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching

Sarah R. Needham ,

Contributed equally to this work with: Sarah R. Needham, Michael Hirsch, Daniel J. Rolfe

Affiliation Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, United Kingdom
⨯
Michael Hirsch ,

Contributed equally to this work with: Sarah R. Needham, Michael Hirsch, Daniel J. Rolfe

Affiliation Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, United Kingdom
⨯
Daniel J. Rolfe ,

Contributed equally to this work with: Sarah R. Needham, Michael Hirsch, Daniel J. Rolfe

Affiliation Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, United Kingdom
⨯
David T. Clarke,

Affiliation Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, United Kingdom
⨯
Laura C. Zanetti-Domingues,

Affiliation Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, United Kingdom
⨯
Richard Wareham,

Affiliation Department of Engineering, University of Cambridge, Cambridge, United Kingdom
⨯
Marisa L. Martin-Fernandez

* E-mail: marisa.martin-fernandez@stfc.ac.uk

Affiliation Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, United Kingdom
⨯

Measuring EGFR Separations on Cells with ∼10 nm Resolution via Fluorophore Localization Imaging with Photobleaching

Sarah R. Needham,
Michael Hirsch,
Daniel J. Rolfe,
David T. Clarke,
Laura C. Zanetti-Domingues,
Richard Wareham,
Marisa L. Martin-Fernandez

Published: May 1, 2013
https://doi.org/10.1371/journal.pone.0062331

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