A Novel Multiplex Tetra-Primer ARMS-PCR for the Simultaneous Genotyping of Six Single Nucleotide Polymorphisms Associated with Female Cancers

Chen Zhang; Ying Liu; Brian Z. Ring; Kai Nie; Mengjie Yang; Miao Wang; Hongwei Shen; Xiyang Wu; Xuejun Ma

doi:10.1371/journal.pone.0062126

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Open Access

Peer-reviewed

Research Article

A Novel Multiplex Tetra-Primer ARMS-PCR for the Simultaneous Genotyping of Six Single Nucleotide Polymorphisms Associated with Female Cancers

Chen Zhang ,

Contributed equally to this work with: Chen Zhang, Ying Liu

Affiliation Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Ying Liu ,

Contributed equally to this work with: Chen Zhang, Ying Liu

Affiliations Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, Department of Food Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou, China
⨯
Brian Z. Ring,

Affiliation Institute of Genomic and Personalized Medicine, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
⨯
Kai Nie,

Affiliation Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Mengjie Yang,

Affiliation Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Miao Wang,

Affiliation Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Hongwei Shen,

Affiliation Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Xiyang Wu ,

* E-mail: maxj2004@yahoo.com.cn (XM); tkentwu@jnu.edu.cn (XW)

Affiliation Department of Food Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou, China
⨯
Xuejun Ma

* E-mail: maxj2004@yahoo.com.cn (XM); tkentwu@jnu.edu.cn (XW)

Affiliation Key Laboratory for Medical Virology, Ministry of Health-National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯

A Novel Multiplex Tetra-Primer ARMS-PCR for the Simultaneous Genotyping of Six Single Nucleotide Polymorphisms Associated with Female Cancers

Chen Zhang,
Ying Liu,
Brian Z. Ring,
Kai Nie,
Mengjie Yang,
Miao Wang,
Hongwei Shen,
Xiyang Wu,
Xuejun Ma

Published: April 17, 2013
https://doi.org/10.1371/journal.pone.0062126

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Polymerase chain reaction
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Single nucleotide polymorphisms
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Genotyping
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Variant genotypes
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Breast cancer
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Gynecologic cancers
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Direct sequencing
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Cervical cancer
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