A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

Karamjit Singh Dolt; Melanie L. Lawrence; Eve Miller-Hodges; Joan Slight; Anna Thornburn; Paul S. Devenney; Peter Hohenstein

doi:10.1371/journal.pone.0062054

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Research Article

A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

Karamjit Singh Dolt ,

Contributed equally to this work with: Karamjit Singh Dolt, Melanie L. Lawrence

Affiliations MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom, The Roslin Institute, University of Edinburgh, Midlothian, United Kingdom
⨯
Melanie L. Lawrence ,

Contributed equally to this work with: Karamjit Singh Dolt, Melanie L. Lawrence

Affiliation MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom
⨯
Eve Miller-Hodges,

Affiliation MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom
⨯
Joan Slight,

Affiliation MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom
⨯
Anna Thornburn,

Affiliation MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom
⨯
Paul S. Devenney,

Affiliation MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom
⨯
Peter Hohenstein

* E-mail: peter.hohenstein@roslin.ed.ac.uk

Affiliations MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom, The Roslin Institute, University of Edinburgh, Midlothian, United Kingdom
⨯

A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

Karamjit Singh Dolt,
Melanie L. Lawrence,
Eve Miller-Hodges,
Joan Slight,
Anna Thornburn,
Paul S. Devenney,
Peter Hohenstein

Published: April 29, 2013
https://doi.org/10.1371/journal.pone.0062054

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