A Genetic Screen Using the PiggyBac Transposon in Haploid Cells Identifies Parp1 as a Mediator of Olaparib Toxicity

Stephen J. Pettitt; Farah L. Rehman; Ilirjana Bajrami; Rachel Brough; Fredrik Wallberg; Iwanka Kozarewa; Kerry Fenwick; Ioannis Assiotis; Lina Chen; James Campbell; Christopher J. Lord; Alan Ashworth

doi:10.1371/journal.pone.0061520

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Open Access

Peer-reviewed

Research Article

A Genetic Screen Using the PiggyBac Transposon in Haploid Cells Identifies Parp1 as a Mediator of Olaparib Toxicity

Stephen J. Pettitt,

Affiliations The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom, CRUK Gene Function Laboratory, The Institute of Cancer Research, London, United Kingdom
⨯
Farah L. Rehman,

Affiliation The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
Ilirjana Bajrami,

Affiliations The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom, CRUK Gene Function Laboratory, The Institute of Cancer Research, London, United Kingdom
⨯
Rachel Brough,

Affiliations The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom, CRUK Gene Function Laboratory, The Institute of Cancer Research, London, United Kingdom
⨯
Fredrik Wallberg,

Affiliation The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
Iwanka Kozarewa,

Affiliation Tumour Profiling Unit, The Institute of Cancer Research, London, United Kingdom
⨯
Kerry Fenwick,

Affiliation Tumour Profiling Unit, The Institute of Cancer Research, London, United Kingdom
⨯
Ioannis Assiotis,

Affiliation Tumour Profiling Unit, The Institute of Cancer Research, London, United Kingdom
⨯
Lina Chen,

Affiliation Tumour Profiling Unit, The Institute of Cancer Research, London, United Kingdom
⨯
James Campbell,

Affiliations The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom, CRUK Gene Function Laboratory, The Institute of Cancer Research, London, United Kingdom, Tumour Profiling Unit, The Institute of Cancer Research, London, United Kingdom
⨯
Christopher J. Lord ,

* E-mail: Chris.Lord@icr.ac.uk (CJL); Alan.Ashworth@icr.ac.uk (AA)

Affiliations The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom, CRUK Gene Function Laboratory, The Institute of Cancer Research, London, United Kingdom
⨯
Alan Ashworth

* E-mail: Chris.Lord@icr.ac.uk (CJL); Alan.Ashworth@icr.ac.uk (AA)

Affiliations The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom, CRUK Gene Function Laboratory, The Institute of Cancer Research, London, United Kingdom
⨯

A Genetic Screen Using the PiggyBac Transposon in Haploid Cells Identifies Parp1 as a Mediator of Olaparib Toxicity

Stephen J. Pettitt,
Farah L. Rehman,
Ilirjana Bajrami,
Rachel Brough,
Fredrik Wallberg,
Iwanka Kozarewa,
Kerry Fenwick,
Ioannis Assiotis,
Lina Chen,
James Campbell

Published: April 25, 2013
https://doi.org/10.1371/journal.pone.0061520

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Transposable elements
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Cloning
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Genetic screens
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Drug screening
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Library screening
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Transposon mutagenesis
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Small interfering RNA
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Toxicity
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