Effective Identification of Akt Interacting Proteins by Two-Step Chemical Crosslinking, Co-Immunoprecipitation and Mass Spectrometry

Open Access

Peer-reviewed

Research Article

Bill X. Huang,

Affiliation Laboratory of Molecular Signaling, National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, United States of America
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Hee-Yong Kim

* E-mail: hykim@nih.gov

Affiliation Laboratory of Molecular Signaling, National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, United States of America
⨯

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Cross-linking
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Immunoprecipitation
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Co-immunoprecipitation
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Cell binding
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Protein interactions
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Lysine
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Transcription factors
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Tandem mass spectrometry
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