Complete Solubilization and Purification of Recombinant Human Growth Hormone Produced in Escherichia coli

Min-Ji Kim; Hyun Soo Park; Kyung Hye Seo; Hyo-Jin Yang; Sook-Kyung Kim; Jun-Hyuk Choi

doi:10.1371/journal.pone.0056168

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Open Access

Peer-reviewed

Research Article

Complete Solubilization and Purification of Recombinant Human Growth Hormone Produced in Escherichia coli

Min-Ji Kim ,

Contributed equally to this work with: Min-Ji Kim, Hyun Soo Park

Affiliations Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 1 Doryong-dong, Youseong-gu, Daejeon, South Korea, Department of Bio-Analytical Science, University of Science & Technology, Daejion, South Korea
⨯
Hyun Soo Park ,

Contributed equally to this work with: Min-Ji Kim, Hyun Soo Park

Affiliation Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 1 Doryong-dong, Youseong-gu, Daejeon, South Korea
⨯
Kyung Hye Seo,

Affiliation Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 1 Doryong-dong, Youseong-gu, Daejeon, South Korea
⨯
Hyo-Jin Yang,

Affiliations Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 1 Doryong-dong, Youseong-gu, Daejeon, South Korea, Department of Bio-Analytical Science, University of Science & Technology, Daejion, South Korea
⨯
Sook-Kyung Kim,

Affiliations Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 1 Doryong-dong, Youseong-gu, Daejeon, South Korea, Department of Bio-Analytical Science, University of Science & Technology, Daejion, South Korea
⨯
Jun-Hyuk Choi

* E-mail: junchoi@kriss.re.kr

Affiliation Center for Bioanalysis, Department of Metrology for Quality of Life, Korea Research Institute of Standards and Science, 1 Doryong-dong, Youseong-gu, Daejeon, South Korea
⨯

Complete Solubilization and Purification of Recombinant Human Growth Hormone Produced in Escherichia coli

Min-Ji Kim,
Hyun Soo Park,
Kyung Hye Seo,
Hyo-Jin Yang,
Sook-Kyung Kim,
Jun-Hyuk Choi

Published: February 7, 2013
https://doi.org/10.1371/journal.pone.0056168

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Protein extraction
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