A Simple and Robust Vector-Based shRNA Expression System Used for RNA Interference

Open Access

Peer-reviewed

Research Article

Xue-jun Wang ,

Contributed equally to this work with: Xue-jun Wang, Ying Li

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Ying Li ,

Contributed equally to this work with: Xue-jun Wang, Ying Li

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
⨯
Hai Huang,

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Xiu-juan Zhang,

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Pei-wen Xie,

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Wei Hu,

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Dan-dan Li,

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Sheng-qi Wang

* E-mail: sqwang@bmi.ac.cn

Affiliation Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People's Republic of China
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Oligonucleotides
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Cloning
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Transfection
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Plasmid construction
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RNA interference
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Small interfering RNA
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Hepatitis B virus
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Luciferase assay
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