Expanding the Repertoire of Gene Tools for Precise Manipulation of the Clostridium difficile Genome: Allelic Exchange Using pyrE Alleles

Yen Kuan Ng; Muhammad Ehsaan; Sheryl Philip; Mark M. Collery; Clare Janoir; Anne Collignon; Stephen T. Cartman; Nigel P. Minton

doi:10.1371/journal.pone.0056051

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Open Access

Peer-reviewed

Research Article

Expanding the Repertoire of Gene Tools for Precise Manipulation of the Clostridium difficile Genome: Allelic Exchange Using pyrE Alleles

Yen Kuan Ng ,

Contributed equally to this work with: Yen Kuan Ng, Muhammad Ehsaan

Affiliation Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
⨯
Muhammad Ehsaan ,

Contributed equally to this work with: Yen Kuan Ng, Muhammad Ehsaan

Affiliation Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
⨯
Sheryl Philip,

Affiliation Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
⨯
Mark M. Collery,

Affiliation Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
⨯
Clare Janoir,

Affiliation Université Paris-Sud, Faculté de Pharmacie, Département de Microbiologie, Unité EA 40-43, Châtenay-Malabry, France
⨯
Anne Collignon,

Affiliation Université Paris-Sud, Faculté de Pharmacie, Département de Microbiologie, Unité EA 40-43, Châtenay-Malabry, France
⨯
Stephen T. Cartman,

Affiliation Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
⨯
Nigel P. Minton

* E-mail: Nigel.Minton@nottingham.ac.uk

Affiliation Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
⨯

Expanding the Repertoire of Gene Tools for Precise Manipulation of the Clostridium difficile Genome: Allelic Exchange Using pyrE Alleles

Yen Kuan Ng,
Muhammad Ehsaan,
Sheryl Philip,
Mark M. Collery,
Clare Janoir,
Anne Collignon,
Stephen T. Cartman,
Nigel P. Minton

Published: February 6, 2013
https://doi.org/10.1371/journal.pone.0056051

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Plasmid construction
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